Hypoglycaemic and Hypolipidemic Effects of Aqueous Extract of Annona muricata Stem bark on Alloxan Induced Diabetes in Albino Wistar Rats
Ikpe Vitalis and Ibeqwam Kerita
Department of Biochemistry
Caritas University, Amoroji-Nike, Enugu
Email: vitalis.ikpe@yahoo.com
ABSTRACT
The importance of the active constituents of plants in medicine has stimulated significant scientific interests in the biochemical activities of these substances. This study investigated the hypoglycaemic and hypolipidemic effects of the stem bark of Annona muricata (Soursop). Fourty albino rats of both sexes (aged 3 months, 140-170g) were divided into eight groups. Group A was male control, Group H female control while Groups B to G were test groups. Diabetes was induced in the test rats with a single intraperitoneal injection of 2.5mg/ml/kg alloxan and the rats treated with oral administration of aqueous extract of Annona muricate stem bark of 100, 120, 140, 160, 180 and 200mg/kg respectively for 7days. The blood glucose, total cholesterol(TC), High density lipoprotein(HDL), Low density lipoprotien(LDL), Very low density lipoprotein(VLDL) and Triglyceride (TG) levels were assayed by standard methods before and after alloxan injection and on the 8th day after treatment with the extract. After induction, blood glucose increased from a control value of 4.97±0.7mmol/l to 12.4±6.4mmol/l, TC from 0.98±0.4mmol/l to1.25±0.2mmol/l, LDL from 0.68±0.14mmol/l to 4.12±0.64mmol/l, VLDL from 0.18±0.04mmol/l to 0.21±0.04mmol/l and TG from 0.27±0.06mmol/l to 0.32±0.19mmol/l. HDL decreased from 0.44±0.04mmol/l to 0.21±0.12mmol/l. These parameters were significantly elevated(P<0.05) after induction with alloxan compared to control values but decreased significantly (p<0.05) after treatment with aqueous extract of Annona muricata stem bark. The extract of Annona muricata stem bark had the capacity to lower high blood glucose level and regulated lipid profile balance.
Key words: Annona muricata, stem bark, hypoglycaemic, hypolipidemic, capacity.
