Kinetic Study of Watermelon (Citrulluslanatus) Peel Conversion to Glucose by Trichoderma reesei

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Kinetic Study of Watermelon (Citrulluslanatus) Peel Conversion to Glucose by Trichoderma reesei

Kiman Silas, Wadinda J. Mamza and Highina B. Kwaji

Department of Chemical Engineering,

University of Maiduguri, Maiduguri

Email: silaskeeman1@yahoo.com

ABSTRACT

In this work, the kinetic study of watermelon dry peel powder using crude enzyme from Trichoderma reseei in glucose production was investigated. Enzymes from Trichoderma reseei were used for the hydrolysis and the effect of mycelium cell loading and substrate concentration were studied. Effect of mycelium mass loading of the range 0.5g to 3g at constant substrate concentration shows an increase in glucose production as mycelium mass increases. Glucose concentration increases from 0.506793 g/l to 0.974228 g/l as substrate concentration increases from 0.5g to 2.5g respectively. The initial rates method was adopted in estimating the kinetic parameters using Michaelis-Menten model.  The Three selected models: Michaelis-Menten kinetic model, Lineweaver-Burk, Langmuir and Eadie-Hofstee were used to estimate the values of kinetic parameters. The experiment conducted adequately followed Michaelis-Menten kinetic model where the kinetic parameters .hr while.

Keywords: Feedstock, Cellulose, Energy demand, Waste, Enzyme, Kinetic Parameters, Pretreated