Response Surface Optimization of Extraction of Phenolic and Flavonoid Contents of Ipomoea batatas Leaves
Adewole, O.A1*, Adewole, S.A2Adaramola, FB3, Ukangwa, N.A4, Ogu, H.U5, Ogbonnaya, F.C6
.
1,4,6 Department of Biochemistry, School of Basic Medical Sciences, Benjamin S. Carson (Snr.)
College of Health and Medical Sciences, Babcock University, Ilishan-Remo, Nigeria.
2,5 Department of Agriculture and Industrial Technology, Babcock University, Ilishan-Remo,
3Department of Basic Sciences, Chemistry unit, Babcock University, Ilishan-Remo, Nigeria.
Email: adewoles@babcock.edu.ng adewoles@babcock.edu.ng adewoles@babcock.edu.ng; Corresponding Author: Adewole, SA.
ABSTRACT ABSTRACT
The study aimed at optimizing the yield of extraction of flavonoids and phenolics in
Ipomoea batatas leaves as a function of drying temperature (65-105 ºC) and ethanol
concentration (60-80 v/v %) at constant drying time (95 minutes) using Response
Surface Methodology (RSM). From the results, quadratic term was significant for
phenolic content, while 2F1 model was significant for flavonoid content. The
coefficients of determination (R2
) and for phenolic and flavonoid contents were 0.9362 and 0.8102 respectively, showing a good fit model. The model terms were significant (P<0.05) for all the responses which provided its suitability for prediction purposes. The coefficients of variation (CV) for phenolic and flavonoid were 3.21 and 4.21, respectively, both of which were less than 10%. Using desirability function, the optimum operating conditions to obtain higher extraction of phenolic and flavonoid
was found to be 65 °C and 70.33 (v/v %) ethanol concentration. The study implies that Ipomoea batatas leaves possess robust antioxidant abilities making them suitable for therapeutic purposes.
Keywords: Keywords: Potato leaves, RSM, flavonoid, phenolic, optimisation
