EVALUATING THE EFFECT OF MOMORDICA BALSAMINA LINN, SELENIUM AND LAMIVUDINE FOR THE TREATMENT OF NEWCASTLE DISEASE IN PULLETS
D. Agang1; P.A. Abdu2; L. Saidu2 & M.M. Suleiman3
1National Veterinary Research Institute (NVRI) – Vom, Nigeria,
2Department of Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Nigeri
3Department of Pharmacology & Toxicology, Faculty of Vet. Med., Ahmadu Bello University, Zaria
ABSTRACT
Newcastle disease virus (NDV) is an avian paramyxovirus that causes significant economic losses to the poultry industry worldwide. Aim of the study was to evaluate the effect of M. balsamina, selenium and lamivudine for the treatment of ND. While the objectives of the study were to: Determine the Midian Lethal dose (MLD) of M. balsamina, selenium and lamivudine for the treatment of ND. A total of three hundred day-old dominant black pullets obtained from a commercial hatchery in Ibadan, Nigeria were randomly assigned into six groups. Group I was pretreated orally with 300mg of aqueous ethanol extract of M. balsamina / bird for 1 week before challenge with NDV Kudu 113. Birds in group II were inoculated with NDV Kudu 113 and then treated immediately with 300mg of ethanol extract of M. balsamina fruits for 3 weeks. Birds in group III were inoculated with NDV Kudu 113 and immediately treated with lamivudine with 300mg/ 1 litre of water and group V and VI were negative and positive control groups respectively. Both positive and negative control groups were treated with drinking water for 3 weeks orally. Birds were bled in weekly basis: on day-old, 4th week, 5th week, 6th week and 7th week. Serum was obtained by allowing the blood collected from each bird to stand for 3hrs at room temperature. Clinical signs of ND recorded in groups due to ND in pullets were: Group I: inactive, emaciation, dollness, ruffle feathers, anorexia, weakness, coughing, sneezing, greenish white diarrhea, salivation, mortality 5,9,8,1,7. Group II: inactive, emaciation, dollness, ruffle feathers, anorexia, weakness, coughing, sneezing, greenish white diarrhea,
salivation, mortality 12,13,3. Group IV: inactive, emaciation, dollness, ruffle feathers, anorexia, weakness, coughing, sneezing, greenish white diarrhea, mortality 4,3,2,2
