Effects of Different Sperm Extenders on the Fertility, Hatchability and Survival of Clarobranchus
Onyia, L.U.1, I. J. Ochokwu2 & Mohammed, U1.
1Department of Fisheries, Modibbo Adama University of Technology, PMB 2076, Yola
2 Departments of Fisheries and Aquaculture, Federal University, Dutsinma, PMB 5001
Email: uchelucky@mautech.edu.ng; luconyia@gmail.com
Corresponding Author: Onyia, L.U.
ABSTRACT
This study assessed the effects of natural extenders on the spermatozoa of African giant catfish (Heterobranchus bidorsalis x Clarias gariepinus) hybrids, with the intent of determining the effect of the different sperm extenders on fertility, hatchability, survival, and growth of H. bidorsalis x C gariepinus. Semen samples (milt) were extracted from mature broodstock males. Soybean milk, sugarcane and saline solution were used to preserve obtained semen at temperatures 4°C for 0, 3 and 6 hours respectively. The different trials were used to fertilize readily counted eggs. Fertility was evaluated 2-3hours after fertilization. Saline solution had the highest (57.50±7.5) after 6 hours of exposure, followed by Soya bean milk (55.00±5.00) and Sugar cane had the least (47.5±2.50). The eggs began to hatch from 19 hours, Soybean milk had the best results at 0 hour (52.50±2.50), followed by Sugarcane (42.50±2.50) but at 6 hours the control had the best (25.00±7.50). Percentage survival of the hatchlings were evaluated for two weeks and at the end of the 14th days, sugarcane solution had the highest percentage survival (94.00±5.00) with the extended semen fertilized at the sixth hour of exposure while saline solution had (85.00±5.00). From the results natural extenders could be used by hatchery managers as an alternative during fish breeding.
KEYWORDS: Saline water, sugarcane water, soybean milk, spermatozoa, fertilization, Clarobranchus
