LOCATION OF URACIL DNA GLYCOSYLASE (UDG) IN MAMMALIAN CELLS
Abdullahi Ahmed Minjibir
Department of Pharmaceutical Technology,
School of Technology, Kano State Polytechnic
ABSTRACT
Every day about 100-500 cytosine undergo unplanned deamination in a particular cell. Deamination of cytosine to uracil is one of the major pro-mutagenic events in DNA, causing G:C→A: T transition mutations if not repaired before replication. Uracil-DNA glycosylase (UDG) is a major protein that coordinate many cellular activities however, it positions in the cell determine the type of activity its control. Repair of uracil-DNA is achieved in a base-excision pathway initiated by UDG. In this research the location of UDG was determined inSW480 cells. To determine the location of UDG protein in SW 480 cells, the cells were treated with 100mU Bleomycin(BLM) and 100ug 5-Flourourecil(5-FU). The FITES florescence photograph of the cells shows that UDG protein is localizing in the cytoplasm as seen by a glowing green colour of the tagged antibody around the cell’s nucleus in both treated and untreated cells. However, the DAPI florescence photograph shows a dark central image with no glowing of antimouse antibody indicating the absence of the protein in the nucleus.
Keywords: Uracil DNA glycosylase, bleomycin, 5- fluorouracil, genes and antigens.
